The use of agar alternatives in cypress in vitro culture (Cupressus sempervirens var. horizontalis)

Document Type : Scientific article

Authors

1 M.Sc. Student of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, I.R. Iran.

2 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, I.R. Iran.

3 Assistant Professor, Department of Soil Science, Faculty of Natural Resources, Yazd University, Yazd, I.R. Iran.

Abstract

In this article, possibility of using of plant seeds of Lallemantia, Sand plantain and chia also tragacnth (50, 100 and 150 g/l), starch of wheat and corn (100, 150 and 200 g/l) instead of agar (6, 7 and 8 g/l) in MS medium by 4000 years old of Abarkooh cypress explant (Cupressus sempervirens var. horizontalis) was investigated. After 30 days of shoot explant establishment in culture medium, percentage of browning, degree of greening and the number of new buds were evaluated. According to the results, the highest and the lowest percentage of browning were obtained in 50, 100 and 150 g/l of chia and agar treatment respectively and it did not show significant difference with starch of corn, sand plantain and lallemantia seeds treatments. With increasing the concentration of materials, percentage of browning decreased. The highest degree of greening was observed in 150 g/l lallemantia and sand plantain seeds and 200 g/l starch of corn treatments and it did not show significant difference with agar (6 g/l). The lowest degree of greening was obtained in two concentrations of 150 and 200 g/l of chia seeds treatments. The highest number of buds was observed in agar treatment by concentrations of 6 and 7 g/l. According to the results, among different investigated materials in this paper, starch of corn was the best and it is also cost-effective compared to the agar.

Keywords

References

- Amiri, M. E., 2010. Control of mineral uptake in vitro: mineral solubility and mineral movement in vitro. LAP Lambert Academic Press, 345 p.
- Amiri, M.E., & K. Arzani, 2006. Mineral availability and growth of banana (Musa acuminate var. Dwarf Cavendish) explant under various relative matric potential in vitro. Journal of Food and Agriculture and Environment, 4: 105-109.
- Arulanantham, R., S. Pathmanathan, N. Ravimannan & N. Kularajany, 2012. Alternative culture media for bacterial growth using different formulation of protein sources, Journal of Natural Products and Plant Resources, 2(6): 697-700.
- Asadi, S., M. Omidi & D. Davoodi, 2005. Micro-tubers propagation of potato by low cost alternative materials in in vitro, Journal of Research and Building, 71: 94-101. (In Persian)
- Atrashi, M., E. Tavakoli Dinani & A. Masoumi. 2011. Possibility the use of medicinal plant seeds in culture medium and determination of the optimum condition of their application as gelling agent instead of agar, Journal of Herbal Drugs, 3: 179-185.
- Babbar, S. B. & N. Jain, 1998. Isubgol as an alternative gelling agent in plant tissue culture media, Plant Cell Reports, 17(4): 318-322.
- Babbar, S. B. & S. C. Gupta, 1986. Effect of carbon source on Datura metel microspore embryogenesis and the growth of callus raised from microspore-derived embryos, Biochemistry and Physiology, Pflanz, 181(5): 331-338.
- Batty, N. & J. Dunwell, 1989. Effect of maltose on the response of potato anthers in culture, Plant Cell Tissue and Organ Culture, 18(2): 221-226.
- Bornman, C. H. & T. C. Vogelmann, 1984. Effect of rigidity of gel medium on benzyladenine‐induced adventitious bud formation and vitrification in vitro in Picea abies, Physiologia Plantarum, 61(3): 505-512.
- Dabai, Y. U. & S. Muhammad, 2005. Cassava starch as an alternative to agar-agar in microbiological media, African Journal of Biotechnology, 4(6): 573-574.
- Ghamari Zare, A., M. Sedaghati, M. Emam, M. H. Assareh & Kh. Kiarostami, 2014. Micropropagation of Eucalyptus maculata from Mature Stock by tissue culture, Iranian Journal of Forest and Poplar Research, 21(3): 581-593. (In Persian).
- Ghashghaie, J., F. Brenckmann & B. Saugier, 1991. Effects of agar concentration on water status and growth of rose plants cultured in vitro, Physiology of Plant, 82(1): 73-78.
- Giovanelli, A. & A. De Carlo, 2007. Micropropagation of Mediterranian cypress (Cupressus sempervirens L.). In: Jain, S. M. & H. Haggman (Eds.), Protocols for Micropropagation of Woody Trees and Fruits. Springer, Dordrecht, The Netherlands, pp. 93-105.
- Gopal, J. & K. Iwama, 2007. In vitro screening against water-stress mediated through sorbitol and polyethylene glycol, Plant Cell Report, 26(5): 693-700.
- Gribble, K., J. P. Conroy, P. Holford & P. J. Milham, 2002. In vitro uptake of minerals by Gypsophila paniculata and hybrid eucalypts, and relevance to media mineral formulation, Australian Journal of Botany, 50(6): 713-723.
- Irannejad Parizi, M., H. Akbari, M. Khoshnevis, M. Shams, T. Abedini, M. Raad & A. Rasouli, 2016. Natural nation of Abarkooh cypress. Yazd University press. (In Persian).
- Jain, R., V. Anjaiah & S. B. Babbar, 2005. Guar gum: a cheap substitute for agar in microbial culture media, Letters in Applied Microbiology, 41(4): 345-349.
- Jourgholami, M., A. Deljouei, E. S. Hosseini Ala & Gh. Zahedi-Amiri, 2017. Effect of soil compaction on functional equilibrium and biomass allocation of Cupressus sempervirens L. var. horizontalis, Journal of Forest Research and Development, 3(2): 91-106.
- Khazaei, H. R., M. Sabet Teimouri & F. Najafi, 2007. Investigation on yield and quality of Isabgol (Plantago ovata L.), Iranian Journal of Field Crop research, 5(1): 77-85. (In Persian)
- Kinnersley, A. M. & W. E. Henderson, 1988. Alternative carbohydrates promote differentiation of plant cells, Plant Cell, Tissue and Organ Culture, 15(1): 3-16.
- Mashayekhi, M., M. Amiri & F. Habibi, 2016. Study of growth and mineral uptake of GF677 (peach and almond hybrid) rootstock in response to in vitro various relative matric potential, Journal of plant production research, 22(4): 1-16. (In Persian).
- Montagne, J. F. C., 1837. Centurie de plantes cellulaires exotiques nouvelles, Annales des Sciences Naturelles. (a) Botanique),(2e Ser), 8: 345-370.
- Murashige, T & F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3): 473-497.
- Piri, Kh. & F. Nazarian Firoozabadi, 2001. Plant tissue culture’s guide. Abou Ali Sina University press. (In Persian)
- Sorvari, S. & O. Schifder, 1987. Influence of sucrose and melibiose on barley anther cultures in starch media, Plant Breeding, 99(2): 164-171.
- Strickland, S. G., J. W. Nichol, C. M. McCall & D. A. Stuart, 1987. Effect of carbohydrate source on alfalfa somatic embryogenesis, Plant Science, 48(2): 113-121.
- Verma, D. C. & D. K. Dougall, 1977. Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures, Plant Physiology, 59(1): 81-85.
- Yamasaki, H. & G. Osuga, 1960. Studies on the propagation of Gelidiaceus algae on the ratio of cystocarposporophyte to tetrasporophyte in Gelidium mansii on the artificial, stone bed. Bull, The Japanese Society of Fish, 26: 9-12.
- Zimmerman, R. H., S. V. Bhardwaj, & I. M. Fordham, 1995. Use of starch-gelled medium for tissue culture of some fruit crops, Plant Cell Tissue and Organ Culture, 43(3): 207-213.
Forest Research and Development
Volume 4, Issue 4
March 2019
Pages 489-499

Files

Share

How to cite

Statistics