Document Type : Scientific article
Authors
1
M.Sc. of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I. R. Iran
2
Asistant Professor, Department of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I. R. Iran
3
Asistant Professor, Department of Environment, Faculty of Natural Resources, Tarbiat Modares University, Noor, I. R. Iran
Abstract
Apple (Malus orientalis) has been distributed throughout the Hyrcanian forest from lowland regions to steep and mountainous areas. For evaluation of genetic diversity, leaf materials were collected from 60 individuals of 6 populations. DNA was extracted and studied by six SSR primers: (GD142), (GD12), (GD147), (GD96), (GD100) and (GD162). The results showed that 45 alleles with 0.39 heterozygosity were detected for six primers. The mean of heterozygosity was 0.39 and the lowest (0) and highest amount (1) were observed for (GD100) in “Afratakhte”, “kodir”, “Sourdar”, “Yoush bala”, “Yoush paein” population and for GD162 in “kodir”, “asalem”, “yoush miani” and “yoush paein” populations, respectively. The maximum Nei genetic distance was belonged to “asalem” and “sourdar” populations and the minimum was related to “Yoush bala” and “asalem”. The result of AMOVA indicated that the intra and inter population diversity were 97% and 3%, respectively that indicated significant diversity within population of this species. Because of different habitat conditions and long geographical distance among populations, The low genetic differentiation and similar heterozygosity within populations show high gene flow among populations of Malus orientalis in north of Iran.
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