Evaluation type of explants and season of sampling under different disinfection treatments for the tissu culture of Buxus hyrcana Pojark

Document Type : Scientific article


1 PhD. Student., Silviculture and Forest Ecology, Dept. of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Recourses. Golestan, Iran

2 Gorgan Unversity of Agricultural Sciences and Natural Resource. forest sciences faculty

3 Dept. of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Recourses. Golestan, Iran


The aim of this study was to find the best explants and sampling period under different sterilization treatments in order to achieve a suitable and optimal protocol for tissue culture studies in Buxus sp. Explants were prepared from different parts of the plant, including leaves and stems. At first, detergent disinfection treatments and initial washing with ordinary water containing a few drops of liquid dish for four to five minutes were performed to remove surface contamination. The Explants were flushed with distilled water five times. Then, under laminar Floe sterilization, use 13 different treatments and disinfect five times each treatment with distilled autoclaved leachate and at the end of the appropriate samples (leaf samples of 0.5 to 1 cm Squares and stem samples of 5 to 10 mm) were isolated from plant material and placed on a PDA medium inside autoclaved petri dishes alongside a flame. In order to measure the contamination, cultures in a growth room with a temperature of 26 ± 1 ° C with a light intensity of 1000 to 1500 lux and dark (incubator) were investigated. The results showed that samples of spring season have less microbial contamination than those in the autumn season. On the other hand, leaf samples showed less contamination percentage compared to stem explants.


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