The effects of different plant growth regulators on micropropagation of Juniperus foetidissima Willd.

Document Type : Scientific article

Authors

1 Ph.D. student of Forestry, Faculty of Natural Resources, Urmia University, Iran

2 Associate Professor, Forestry department, Faculty of Agricalture and Natural Resources, Urmia University, Iran

3 Associate Professor, Horticultural Department, Faculty of Agriculture, Urmia University, Iran

4 Urmia University

Abstract

This research was carried out with the aim of optimizing in vitro proliferation of J. foetidissima under various proliferations and rooting treatments. For this purpose, the plant samples were collected of healthy and young annual branches of this species without symptoms of disease and deficiency from Arasbaran forests. This experiment was conducted as a factorial experiment in a completely randomized design and data were analyzed using two-way GLM. In order to test micro propagation, well established explants were treated with BAP, KIN and TDZ growth regulators at 4 levels (0, 1, 3, 5 mg / l) individually or in combination with IAA at three levels (0, 0.1 and 0.5 mg / l). Various parameters such as survival percentages, regeneration percentage, number of shoots and shoot length were considered. Also in order to survey rooting in J. foetidissima, shoots over 1 cm height were cultured in OM, MS, DKW, WPM, 1/2 MS and 1/4 MS media containing IBA (0.5 mg/l) and rooting percentage was recorded. Results revealed that, there was a significant difference at 5% level between different experimental treatments. In BAP and IAA experiments, the highest shoot proliferation (3.82, 2.78 and 1.67 shoots per explant) were obtained in MS medium supplemented with BAP (1 mg/l) and IAA (0.1 mg/l), KIN (3 mg/l) and TDZ (3 mg/l) respectively.

Keywords

References

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Forest Research and Development
Volume 6, Issue 4
January 2021
Pages 573-591

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