بررسی تنوع ژنتیکی نوش (‏Thuja orientalis‏) با استفاده از نشانگر پراکسیداز در ذخیره‌گاه جنگلی سورکش

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران‏

2 دانشیار گروه جنگل‌شناسی و اکولوژی جنگل، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران‏

3 کارشناس اداره کل منابع طبیعی و آبخیزداری استان گلستان، گرگان، ایران‏

چکیده

هدف این پژوهش تعیین و مقایسه تنوع ژنتیکی جمعیت‌های نوش در ذخیره‌گاه جنگلی سورکش به‌‌کمک چند‍شکلی نشانگر بیوشیمیایی پراکسیداز در دو اندام شاخه و فلس بوده است. نمونه‌برداری به‌طور همزمان از فلس و شاخه دوساله 30 پایه نوش از سه جمعیت انجام شد. نمونه‌ها از ارتفاع میانه تاج و از جهت ثابت جنوبی تاج شده شدند. تجزیه و تحلیل داده‌ها به‌کمک نرم‌افزار GenAlEx در خصوص ویژگی‌های آللی نشانگرهای مورد پژوهش و مقایسه توانایی دو اندام و گروه‌بندی پایه‌ها برای بررسی تنوع درون و بین جمعیتی توسط تحلیل خوشه‌ای داده‌های الکتروفورتیکی (ژل‌های تهیه شده) توسط نرم‌افزار NTSYS 2.02 انجام شد. بررسی کیفی با الکتروفورز عمودی به‌روش PAGE (پلی آکریل آمید ژل الکتروفورز) انجام شد و داده‌های الکتروفورتیکی پایه‌های هر منطقه به‌کمک تحلیل خوشه‌ای گروه‌بندی شدند. با توجه به‌نتایج، جمعیت دو با میانگین تعداد آلل مشاهده شده در هر جایگاه ژنی (2)، میانگین تعداد آلل مؤثر (77/1)، هتروزیگوتی قابل انتظار (42/0) و درصد پلی‌مورفیسم (100) دارای بیشترین شاخص‌های تفکیک آللی نسبت به دو جمعیت دیگر است. همچنین تنوع درون جمعیتی (حدودأ 60) بیشتر از تنوع بین جمعیتی (حدودأ 40) به‌دست آمد که باید در مدیریت این ذخیره‌گاه برای افزایش تنوع مدنظر قرار گیرد. همچنین اندام فلس در نوش با میانگین تعداد آلل مشاهده شده در هر جایگاه ژنی (48/1)، میانگین تعداد آلل مؤثر (49/1)، هتروزیگوتی قابل انتظار (27/0)، میانگین شاخص اطلاعات شانون (39/0)، تعداد باند (9) و درصد چندشکلی (67/66) نسبت به ویژگی‌های آللی اندام شاخه دارای مقادیر بیشتری در پژوهش­های تنوع ژنتیکی است.

کلیدواژه‌ها


عنوان مقاله [English]

Study of genetic diversity Thuja orientalis by Peroxidase marker in reserved forest of Sorkesh ‎

نویسندگان [English]

  • Masoumeh Khazaei poul 1
  • Davoud Azadfar 2
  • Zohreh Saeedi 3
1 M.Sc. Graduate of Silviculture and Forest Ecology, Faculty of Natural Resources, Gorgan University of ‎Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran‎
2 Associate Professor, Department of Silviculture and Forest Ecology, Faculty of Natural Resources, Gorgan ‎University of Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran‎
3 Expert of the General Department of Natural Resources and Watershed Management of Golestan ‎Province, Gorgan, I. R. Iran‎
چکیده [English]

The aim of this study was to determine and compare the genetic diversity of wild populations of Thuja orientalis by biochemical markers polymorphism of leaves and two-year-old branches Peroxidase. The sampling was done in 30 individuals of Thuja orientalis in three populations. Samples were taken from medium crown height in the south direction. Data analysis was performed by GenAlEx software on the allele characteristics of the studied markers and comparing the ability of these two organs and grouping of the trees to examine the intra- and inter-population variation by cluster analysis of electrophoretic data (prepared gels) by NTSYS 2.02 software. Qualitative measures was studied by vertical electrophoresis and PAGE (polyacrylamide gel electrophoresis) method and the electrophoretic data were classified using cluster analysis. According to the results, the second population with the average number of alleles observed per gene locus (2), the average effective number of alleles (1.77), the expected heterozygosity (0.42), and the percentage of polymorphism (100) have the most allelic separation indices in comparison to the other two populations. Also, the higher genetic diversity in intra- population (nearly 60) than inter-populations (nearly 40) should be considered in the management of this reserved forest to increase diversity. Also, the scale in Thuja sp. with the average number of alleles observed per gene locus (1/48), the average effective number of alleles (1.49), the expected heterozygosity (0.27), mean Shannon's information index (0.39), number of bands (9) and percentage of polymorphism (66/67) has better allelic characteristics than the branch in genetic diversity studies.

کلیدواژه‌ها [English]

  • Keywords: electrophoresis
  • intra population diversity
  • Fazel Abad
  • Biochemical marker
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