Study of genetic diversity Thuja orientalis by Peroxidase marker in reserved forest of Sorkesh ‎

Document Type : Scientific article

Authors

1 M.Sc. Graduate of Silviculture and Forest Ecology, Faculty of Natural Resources, Gorgan University of ‎Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran‎

2 Associate Professor, Department of Silviculture and Forest Ecology, Faculty of Natural Resources, Gorgan ‎University of Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran‎

3 Expert of the General Department of Natural Resources and Watershed Management of Golestan ‎Province, Gorgan, I. R. Iran‎

Abstract

The aim of this study was to determine and compare the genetic diversity of wild populations of Thuja orientalis by biochemical markers polymorphism of leaves and two-year-old branches Peroxidase. The sampling was done in 30 individuals of Thuja orientalis in three populations. Samples were taken from medium crown height in the south direction. Data analysis was performed by GenAlEx software on the allele characteristics of the studied markers and comparing the ability of these two organs and grouping of the trees to examine the intra- and inter-population variation by cluster analysis of electrophoretic data (prepared gels) by NTSYS 2.02 software. Qualitative measures was studied by vertical electrophoresis and PAGE (polyacrylamide gel electrophoresis) method and the electrophoretic data were classified using cluster analysis. According to the results, the second population with the average number of alleles observed per gene locus (2), the average effective number of alleles (1.77), the expected heterozygosity (0.42), and the percentage of polymorphism (100) have the most allelic separation indices in comparison to the other two populations. Also, the higher genetic diversity in intra- population (nearly 60) than inter-populations (nearly 40) should be considered in the management of this reserved forest to increase diversity. Also, the scale in Thuja sp. with the average number of alleles observed per gene locus (1/48), the average effective number of alleles (1.49), the expected heterozygosity (0.27), mean Shannon's information index (0.39), number of bands (9) and percentage of polymorphism (66/67) has better allelic characteristics than the branch in genetic diversity studies.

Keywords

References

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Forest Research and Development
Volume 7, Issue 3
December 2021
Pages 387-401

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