تنوع ژنتیکی نهال‌های گردو‌ی ایرانی (‏Juglans regia L.‎‏) با استفاده از نشانگرهای ‏SSR

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی دکترای جنگلداری، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران‏

2 دانشیار، گروه جنگلداری، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران. ‏

3 استاد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران‏

4 دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران‏

5 استادیار، گروه محصولات زراعی، دانشکده کشاورزی، دانشگاه یوزونجو یل وان، وان، ترکیه

6 استاد، گروه علوم باغبانی، پردیس ابوریحان، دانشگاه تهران، تهران‏

چکیده

اولین گام برای شناسایی، حفظ و نگهداری ذخایر توارثی و اساس پژوهش­های ژنتیکی و برنامه­های اصلاحی تعیین مقدار تنوع ژنتیکی در مواد گیاهی است. به­کارگیری نشانگرهای مولکولی یکی از ابزارهای بسیار مهم در این زمینه است. در این راستا حفظ تنوع ژنتیکی گیاهان بومی و ارزیابی مقدار تنوع آن­ها ضروری به­نظر می‌رسد. برای ارزیابی تنوع ژنتیکی 140 نهال گردوی ایرانی از 20 جفت آغازگر SSR استفاده شد. 20 مکان SSR در مجموع 138 آلل تولید کرد. کمترین و بیشترین تعداد آلل، به­ترتیب مربوط به مکان­های WGA69، WGA71 و JRHR211298 (پنج آلل) و WGA202 (نه آلل) بود. کمترین و بیشترین تعداد آلل مؤثر به­ترتیب مربوط به مکان­های JRHR211298 و JRHR217037 بود. بیشترین مقدار هتروزیگوتی مشاهده­شده توسط مکان­ WGA69 تولید شد. همۀ مکان­های SSR در جمعیت مورد بررسی انحراف معنی­داری (001/0p≤) از تعادل هاردی-­واینبرگ نشان دادند. دندروگرام حاصل از روش Neigbour-joining، 140 نهال گردو را در 5 گروه عمده قرار داد. بررسی ساختار جمعیت با نرم­افزار Structure 2.3.1، 2 گروه (2k=) احتمالی را شناسایی کرد. که نتایج هردو تا حد زیادی با هم هم­خوانی داشتند. اطلاعات حاصل بر اساس ماتریس سهم عضویت افراد و مقدار Fst نشان داد که تمایز قابل­ملاحظه­ای بین گروه­های احتمالی وجود ندارد.

کلیدواژه‌ها

عنوان مقاله [English]

Genetic diversity in Persian walnut (Juglans regia L.) seedlings using SSR markers

نویسندگان [English]

  • َََAyda Taheri 1
  • Nasrin Seyedi 2
  • Babak Abdollahi Mandoulakani 3
  • Ghader Mirzaghaderi 4
  • ُSolmaz Najafi 5
  • Kourosh Vahdati 6
1 Ph.D. student of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I.R. Iran.‎
2 Associate Professor, Department of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I.R. ‎Iran. ‎
3 Professor, Department of Plant Production Genetics, Faculty of Agriculture, Urmia University‌, Urmia, I.R. ‎Iran. ‎
4 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, I.R. Iran. ‎
5 Assistant Professor, Department of Field Crops, Faculty of Agriculture, Van Yuzuncu Yil University, Van, ‎Turkey. ‎
6 Professor, Department of Horticulture, College of Aburaihan, Pakdasht, Tehran University, I.R. Iran. ‎
چکیده [English]

The first and basic step in identification, conservation, protection of gene resources, genetic research, and breeding programs is determination of genetic diversity in plant material. Using of molecular markers is one of the most important tools in this field. Therefore, the conservation and evaluation of genetic diversity of native plants is necessary. In order to evaluate the genetic diversity of 140 Iranian walnut seedlings, 20 SSR primer pairs were used. The used SSR loci generated 138 alleles. The minimum and maximum number of alleles were for SSR loci WGA69, WGA71, JRHR211298 (five alleles) and WGA202 (nine alleles), respectively. Loci JRHR211298 and loci JRHR217037 produced the minimum and maximum number of effective alleles, respectively. The most value of observed heterozygosity (Ho) generated by loci WGA69. All used SSR loci, showed deviation (P≤0.001) from Hardy–Weinberg. Neigbour-joining dendrogram divided 140 walnut seedlings into five main groups. The study of population structure, with Structure 2.3.1 software, identified two possible groups (k=2). The results of both were largely consistent. The data based on the individual membership share matrix and the Fst rate showed that there was no significant difference between two possible groups.

کلیدواژه‌ها [English]

  • Cluster analyses
  • Hardy–Weinberg equilibrium
  • Observed heterozygosity
  • Structure software

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پژوهش و توسعه جنگل
دوره 8، شماره 1
خرداد 1401
صفحه 13-26

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