تأثیر قارچ میکوریز و ریزوباکتری محرک رشد بر فعالیت آنزیم‌های آنتی‌اکسیدانی نهال استبرق تحت تنش خشکی

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دکترای جنگلداری، دانشکده منابع طبیعی و علوم دریایی نور، دانشگاه تربیت مدرس، نور، ایران

2 استادیار گروه جنگلداری، دانشکده کشاورزی و منابع طبیعی اهر، دانشگاه تبریز، اهر، ایران

3 استاد گروه جنگلداری، دانشکده منابع طبیعی و علوم دریایی نور، دانشگاه تربیت مدرس، نور، ایران

چکیده

این پژوهش با هدف بررسی فعالیت آنزیم­های آنتی‌اکسیدان مانند کاتالاز، سوپراکسیددیسموتاز، آسکوربات و پراکسیداز در نهال استبرق (Calotropis procera Ait) تلقیح یافته با میکروارگانیسم­های میکوریزی و ریزوباکتریایی تحت تأثیر تنش خشکی در یک شرایط گلخانه­ای طی یک دوره شش‌ماهه انجام شد. آزمایش در سه سطح تلقیحی (شاهد یا عدم تلقیح، قارچ میکوریز رﯾﺰوﻓﺎﮔﻮس اﯾﺮﮔﻮﻻرﯾﺲ و ریزوباکتریایی سودوموناس پوتیدا) و با سه سطح تنش خشکی دوره­ای (سه، شش و 12 روز آبیاری) در قالب طرح پایه کاملاً تصادفی با سه تکرار انجام شد. نتایج نشان داد که تلقیح میکوریز و ریزوباکتریایی سودوموناس تحت شرایط خشکی به­طور معنی­داری فعالیت آنزیم­های آنتی­اکسیدان را افزایش داد. بیشترین فعالیت آنزیم کاتالاز (57/1 واحد آنزیمی بر میلی­گرم وزن تر)، سوپراکسید دیسموتاز (43/17 واحد آنزیمی بر میلی­گرم وزن تر) و آسکوربات پراکسیداز (56/4 واحد آنزیمی بر میلی­گرم وزن تر) در نهال­های میکوریزی تحت تنش خشکی 12 روز و همچنین بیشترین مقدار فعالیت آنزیم پراکسیداز در نهال میکوریزی (96/11 واحد آنزیمی بر میلی­گرم وزن تر) و ریزوباکتریایی (9/12 واحد آنزیمی بر میلی­گرم وزن تر) در شرایط خشکی 12 روز مشاهده شد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of mycorrhizal fungi and growth-promoting rhizobacteria on the activity of antioxidant enzymes of Calotrope seedlings under drought stress

نویسندگان [English]

  • Ehsan Ghanbary 1
  • Omid Fathizadeh 2
  • Masoud Tabari 3
1 Ph.D. of Forestry, Department of Forestry, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, I.R. Iran
2 Department of forestry, Ahar faculty of agriculture and natural resources, University of Tabriz, Ahar, Iran
3 Professor, Department of Forestry, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, I.R. Iran
چکیده [English]

This study aimed to investigate the antioxidant enzyme activities such as catalase, superoxide dismutase, ascorbate peroxidase in Calotrope seedlings (Calotropis procera Ait) inoculated with mycorrhizal and Rhizobacterial microorganisms affected by drought stress under greenhouse conditions during a six-month period. Experiment in three inoculation levels (non-inoculated or control, Rhizophagus irregularis mycorrhizal fungi and Pseudomonas putida Rhizobacterial) and with three levels of drought durations (3, 6, and 12 days irrigation) in a completely randomized design with three replications was conducted. Results revealed that inoculation of mycorrhiza and Pseudomonas Rhizobacterial under drought stress significantly increased the activity of antioxidant enzymes. Maximum activity of catalase (1.57 units of enzyme per mg of fresh weight), superoxide dismutase (17.43 units of enzyme per mg of fresh weight) and ascorbate peroxidase (4.56 units of enzyme per mg of fresh weight) in mycorrhizal seedlings under drought stress for 12 days and also highest amount of peroxidase activity in mycorrhizal (11.96 units of enzyme per mg of fresh weight) and Rhizobacterial (12.9 units of enzyme per mg of fresh weight) seedlings under drought conditions for 12 days were observed.

کلیدواژه‌ها [English]

  • Active oxygen species
  • Oxidative stress
  • Pseudomonas
  • Rhizophagus irregularis

مراجع

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پژوهش و توسعه جنگل
دوره 6، شماره 3
آذر 1399
صفحه 477-489

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