نوع مقاله : علمی - پژوهشی
نویسندگان
1 دانشجوی دکتری گروه جنگلداری، دانشکده منابع طبیعی دانشگاه ارومیه، ایران
2 دانشیار، گروه جنگلداری، دانشکده کشاورزی و منابع طبیعی دانشگاه ارومیه، ایران.
3 دانشیار گروه علوم باغبانی، دانشکده کشاورزی دانشگاه ارومیه، ایران
4 دانشیار، دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
This research was carried out with the aim of optimizing in vitro proliferation of J. foetidissima under various proliferations and rooting treatments. For this purpose, the plant samples were collected of healthy and young annual branches of this species without symptoms of disease and deficiency from Arasbaran forests. This experiment was conducted as a factorial experiment in a completely randomized design and data were analyzed using two-way GLM. In order to test micro propagation, well established explants were treated with BAP, KIN and TDZ growth regulators at 4 levels (0, 1, 3, 5 mg / l) individually or in combination with IAA at three levels (0, 0.1 and 0.5 mg / l). Various parameters such as survival percentages, regeneration percentage, number of shoots and shoot length were considered. Also in order to survey rooting in J. foetidissima, shoots over 1 cm height were cultured in OM, MS, DKW, WPM, 1/2 MS and 1/4 MS media containing IBA (0.5 mg/l) and rooting percentage was recorded. Results revealed that, there was a significant difference at 5% level between different experimental treatments. In BAP and IAA experiments, the highest shoot proliferation (3.82, 2.78 and 1.67 shoots per explant) were obtained in MS medium supplemented with BAP (1 mg/l) and IAA (0.1 mg/l), KIN (3 mg/l) and TDZ (3 mg/l) respectively.
کلیدواژه‌ها [English]
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